CURRICULUM VITAE

[nom] DATA

Name: · [nom]

Citizenship: · Hellenic

e-mail: · [e-mail], [e-mail]

EDUCATION

1999-2007: PhD in Molecular Biology – Biomedicine, Faculty of Medicine, Univ. of Crete

1997-1999: Master in Molecular Biology – Biomedicine, Faculty of Medicine, Univ. of Crete

1983-1988: Diploma, Faculty of Chemistry, University of Thessaloniki

WORK EXPERIENCE

2022 – · : Scientific Consultant at Pharmathen Pharmaceuticals

2021 – · : Senior Staff Scientist, Laboratory of Neuroscience, Institute of Molecular Biology &

Biotechnology and Medical School, University of Crete, Crete, Greece

2017 – 2021: Principal Staff Scientist, Laboratory of Neuroscience, Institute of Molecular Biology &

Biotechnology and Medical School, University of Crete, Crete, Greece

2016 – 2021: Teaching staff at the Faculty of Medicine, University of Crete

1999 – 2005: Teaching staff at the Technological Educational Institute of Crete

1991 – 2017: Special Research Assistant, Laboratory of Neuroscience, Institute of Molecular Biology &

Biotechnology and Medical School, University of Crete, Crete, Greece

RESEARCH INTERESTS

My scientific interests focus on the biology of the nervous system and especially on neuronal development and axoglial interactions during myelination.

I have participated in several research projects related to myelination and demyelinating diseases. Since 2018 we have initiated two research projects concerning the use of biobased and biocompatible nanomolecules for drug encapsulation and drug delivery. Our main goal is the use of novel nanoparticles to deliver known agents that could improve remyelination in vivo. In particular, we are currently working on “NANOMYTHiC” - (T2EDK-00501), a program where we employ innovative nanocarriers for the targeted and prolonged treatment of demyelinating diseases of the central nervous system.

During my scientific career I have worked with multiple experimental models, such as chick embryos, mice, and various mammalian neuronal and other cell lines.

My technical expertise includes several molecular and biochemical techniques, like gene cloning, PCR, mouse genetics, protein and mRNA expression analysis (immunohistochemistry/immuno-fluorescence and in situ hybridization respectively), real time PCR, cell culture, tissue cultures, brain slice electroporation, FACS, in utero electroporation etc.

FUNDING/AWARDS

2020-2023: Operational Programme Competitiveness, Entrepreneurship and Innovation. Innovative

Nanocarriers for the targeted and prolonged treatment of cancer and demyelinating diseases of the central nervous system (NANO-MYTHiC). Grant number Τ2ΕΔΚ-00501

2013: EMBO Short Term Fellowship, 3-months visit to the National Institute for Medical Research, Division of Molecular Neurobiology, London. Project Title: The role of Rac1 and Rac3 in interneuron development:

an ex-vivo study

2012: Travelling Fellowship of “Development and The Company of Biologists Limited”, 1-month visit to the National Institute for Medical Research, Division of Molecular Neurobiology, London for training in “brain slices electroporation”

1998 – 1999: M.Sc. Fellowship of Excellence (IKY)

1997 – 1998: M.Sc. Fellowship of Excellence (IKY)

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PUBLICATIONS:

• Kalafatakis, I., Papagianni, F., Theodorakis, K., Karagogeos, D. Nogo-A and LINGO-1:

Two Important Targets for Remyelination and Regeneration. International Journal of Molecular Sciences. 2023, 24(5), 4479; [lien]

• Kounoupa, Z., Tivodar, S., Theodorakis, K., Kyriakis, D., Denaxa, M., Karagogeos, D.

Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneurons. Journal of Cell Science, 2023 February 6: jcs.260373. doi: 10.1242/jcs.260373.

• Chantziou, A., Theodorakis, K., Polioudaki, H., de Bree, E., Kampa, M., Mavroudis, D., Castanas, E., Theodoropoulos, A. P. Glycosylation modulates plasma membrane trafficking of CD24 in breast cancer cells. International Journal of Molecular Sciences, 2021 July 29;22(15): 8165.
• Savvaki, M., Kafetzis, G., Kaplanis, S.I., Ktena, N., Theodorakis, K. and Karagogeos, D.

Neuronal, but not glial Contactin 2, negatively regulates axon regeneration in the injured adult optic nerve. European Journal of Neuroscience, 2021 Mar;53(6):1705-1721.

• Koumentakou, I., Terzopoulou, Z., Michopoulou, A., Kalafatakis, I., Theodorakis, K., Tzetzis, D., Bikiaris, D. Chitosan dressings containing inorganic additives and levofloxacin as potential wound care products with enhanced hemostatic properties. International Journal of Biological Macromolecules, 2020 Nov. 1;162:693-703
• Kastriti, M.E., Stratigi, A., Mariatos, D., Theodosiou, M., Savvaki, M., Kavkova, M., Theodorakis, K., Vidaki, M., Zikmund, T., Kaiser, J., Adameyko, I., Karagogeos, D.

Ablation of CNTN2+ Pyramidal Neurons During Development Results in Defects in Neocortical Size and Axonal Tract Formation. Frontiers in Cellular Neuroscience, Volume

13:454, November 2019.

• Papadogkonaki, S., Theodorakis, K., Thermos, K. Endogenous and synthetic cannabinoids induce the downregulation of cannabinoid CB1 receptor in retina. Experimental Eye Research,Volume 185, August 2019
• Tivodar, S., Kalemaki, K., Kounoupa, Z., Vidaki, M., Theodorakis, K., Denaxa, M., Kessaris, N., de Curtis, I., Pachnis. V., Karagogeos, D. Rac-GTPases Regulate Microtubule Stability and Axon Growth of Cortical GABAergic Interneurons. Cereb Cortex. 2015 Sep;25(9):2370-82.
• Savvaki, M., Theodorakis, K., Zoupi, L., Stamatakis, A., Tivodar, S., Kyriacou, K., Dalezios, Y., Stylianopoulou, F., Karagogeos, D. The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of Tag-1 homozygous mutants. J Neurosci. 20;30(42):[téléphone]).
• Denaxa, M., Kyriakopoulou, K., Theodorakis, K., Trichas, G., Vidaki, M., Takeda, Y., Watanabe, K., Karagogeos, D. The adhesion molecule TAG-1 is required for proper migration of the superficial migratory stream in the medulla but not of cortical interneurons.

Developmental Biology, 288:87-99 (2005).

• Denaxa, M., Pavlou, O., Tsiotra, P., Papadopoulos, G.C., Liapaki, K., Theodorakis, K., Papadaki, C., Karagogeos, D., Papamatheakis, J. The upstream regulatory region of the gene for the human homologue of the adhesion molecule TAG-1 contains elements driving neural specific expression in vivo. Mol. Brain Res. 118:91-101, (2003).

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• Theodorakis, K., Kyriakopoulou, K., Wassef, M. and Karagogeos, D. Novel sites of expression of the bHLH gene Nscl1 in the developing nervous system. Mechanisms of Development ,119 Suppl 1: S103-6, (2002).
• Pavlou, Ο., Theodorakis, Κ., Falk, J., Kutsche, M., Schachner, M., Faivre-Sarrailh, C., and Karagogeos, D. Analysis of interactions of the adhesion molecule TAG-1 and its domains with other immunoglobulin superfamily members. Molecular and Cellular Neuroscience, 20: [téléphone]).
• Tsiotra,P.C., Theodorakis,K., Papamatheakis,J., and Karagogeos,D. The fibronectin domains of the neural adhesion molecule TAX-1 are necessary and sufficient for homophilic binding. J. Biol. Chem. 271:[téléphone], (1996).
• Grigoriou,M., Kastrinaki,M.C., Modi,W.S., Theodorakis,K., Mankoo,B., Pachnis,V. and Karagogeos,D. Isolation of the human MOX 2 homeobox gene and localization to chromosome 7p22.1-p21.3. Genomics 26:550-555, (1995).
• Tsiotra,P.C., Karagogeos,D., Theodorakis,K., Michaelidis,T.M., Modi,W.S., Furley, A.J., Jessell,T.M. and Papamatheakis,J. Isolation of the cDNA and chromosomal localization of the gene (TAX 1) encoding the human axonal glycoprotein TAG-1. Genomics 18:562-567, (1993).
• Kiosseoglou,V., Theodorakis,K., and Doxastakis,G. The rheology of tomato seed protein isolate films at the corn oil-water interface. Colloid Polym. Sci. 267:834-838, (1989).

Posters and oral presentations:

• Ilias Kalafatakis, Fevronia Papagianni, Evi Christodoulou, Ioanna Koumentakou, Kostas Theodorakis, Dimitris Bikiaris, Domna Karagogeos. Testing of a-Lingo-1 and a-Nogo-A and preparation of innovative formulations for their targeted and controlled administration in cuprizone-induced demyelination model. 9th Conference of Hellenic Academy of Neuroimmunology, Thessaloniki 8-11 December, 2022.
• Amanda Chantziou, Hara Polioudaki, Kostas Theodorakis, Elena Gkimprixi, George Notas, Marilena Kampa, Elias Castanas, Panayiotis A. Theodoropoulos. Nuclear localization of CD24 in breast cancer cells. 70th Annual Conference of the Hellenic Society of Biochemistry and Molecular Biology (HSBMB), Athens, Greece (2019)
• Maria Eleni Kastriti, Dimitrios Mariatos, Michaela Kavkova, Kostas Theodorakis, Tomas Zikmund, Josef Kaiser, Igor Adameyko and Domna Karagogeos. Cortical and axonal defects in a novel mouse line with a genetic ablation of CNTN2+ pyramidal neurons.

Hellenic Society for Neuroscience, 28th Annual Μeeting, Heraklion, Greece (2019)

• Maria Savvaki, George Kafetzis, Kostas Theodorakis and Domna Karagogeos. A new player in CNS axon regeneration. Hellenic Society for Neuroscience, 28th Annual Μeeting, Heraklion, Greece (2019)
• Savvaki M., Kafetzis G., Theodorakis K., Karagogeos D. Contactin-2 role in axon regeneration. XIV European Meeting on Glial Cells in Health and Disease, Porto July 10 – 13, 2019

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• Zouzana Kounoupa, Simona Tivodar, Kostas Theodorakis and Domna Karagogeos.

Cytoskeletal dynamics in mice missing the majority of cortical interneurons as a result of Rac1 and Rac3 deficiencies. EMBO Workshop: Cell biology of the neuron: Polarity, plasticity and regeneration. May 2019 | Heraklion, Greece

• Zouzana Kounoupa, Simona Tivodar, Kostas Theodorakis and Domna Karagogeos.

Defects in actin and microtubule dynamics in mice missing Rac1 and Rac3 function. FEBS, Advanced Lecture Course and European Cytoskeletal forum Meeting. 20-24 September

2018, Prague, Czech Republic (2018).

• Simona Tivodar, Kostas Theodorakis, Katerina Kalemaki, Zouzana Kounoupa, Marina Vidaki, Myrto Denaxa, Vassilis Pachnis , and Domna Karagogeos. Rac1 and 3 is necessary in cycling progenitors and postmitotic cells destined to become GABAergic cortical interneurons. 65th Congress of the Hellenic Society of Biochemistry and Molecular Biology, Thessaloniki, Greece. November 28-30 (2014).
• Simona Tivodar, Katerina Kalemaki, Zouzana Kounoupa, Marina Vidaki, Kostas Theodorakis, Myrto Denaxa, Nicoletta Kessaris, Ivan de Curtis, Vassilis Pachnis , and Domna Karagogeos. The absence of Rac1 and Rac3 significantly affects actin-microtubule dynamics in developing cortical interneurons. DENDRITES 2014, FORTH, Crete, Greece, July 1-4, (2014).
• Simona Tivodar, Katerina Kalemaki, Zouzana Kounoupa, Marina Vidaki, Kostas Theodorakis, Myrto Denaxa, Nicoletta Kessaris, Ivan de Curtis, Vassilis Pachnis, and Domna Karagogeos. Rac-GTPases are intracellular mediators of cortical GABAergic interneuron development. 9th FENS Forum of Neuroscience, Milan, Italy, July 5-9 (2014).
• Simona Tivodar, Katerina Kalemaki, Zouzana Kounoupa, Marina Vidaki, Kostas Theodorakis, Myrto Denaxa, Nicoletta Kessaris, Ivan de Curtis, Vassilis Pachnis , and Domna Karagogeos. The absence of Rac1 and Rac3 significantly affects actin-microtubule dynamics in developing cortical interneurons. Cortical Development, Chania, Crete, Greece, May 22-25, (2014).
• Tivodar S., Kalemaki K., Kounoupa Z., Vidaki M., Theodorakis K., Denaxa M., Kessaris N., DeCurtis I., Pachnis V., Karagogeos D. Rac–GTPases regulate microtubule stability and axon growth of cortical GABAergic interneurons. Hellenic Society for Neuroscience, 26th Annual Μeeting, Athens, Greece (2013)
• Savvaki M., Theodorakis K., Zoupi L., Stamatakis A., Tivodar S., Dalezios Y., Stylianopoulou F., Karagogeos D. The Expression of TAG-1 in Glial Cells is Sufficient for the Formation of the Juxtaparanodal Complex and the Phenotypic Rescue of Tag-1 Homozygous Mutants in the CNS. Neuroscience days, Athens (2010)
• Theodorakis K., Savvaki M., Zoupi L., Tivodar S., Karagogeos D. Glial TAG-1 organizes the juxtaparanodal region of myelinated fibers. Hellenic Society of Biochemistry and Molecular Biology, 60th Annual Meeting (2009).
• Karagogeos D., Savvaki M., Theodorakis K., Zoupi L., Tivodar S. Oligodendrocyte- specific expression of the adhesion molecule TAG-1 in the organization of myelinated fibers. Hellenic Society for Neuroscience, 22th Annual Μeeting, Rhodes, Greece (2009).
• Karagogeos D., Savvaki M., Theodorakis K., Zoupi L., Tivodar S. Oligodendrocyte- specific expression of the adhesion molecule TAG-1 in the organization of myelinated fibers. 9th European Meeting on Glial Cells in Health and Disease, Paris (2009).

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• Karagogeos D., Savvaki M., Theodorakis K., Zoupi L., Tivodar S. Oligodendrocyte- specific expression of the adhesion molecule TAG-1 in the organization of myelinated fibers. 2nd ELA Research Foundation Congress, Luxembourg (2009).
• Savvaki M., Zoupi L., Theodorakis K., Tivodar S., Karagogeos The role of glial TAG-1 in the juxtaparanodal organization of CNS myelinated fibers. Keystone Symposia in Multiple Sclerosis, Santa Fe, New Mexico (2009).
• Vidaki, M., Doulgeraki, K., Theodorakis, K., Pachnis, V. and Karagogeos, D. RAC-1 is necessary for the development of GABAergic interneurons in the cortex. Hellenic Society for Neuroscience, 22th Annual Μeeting, (2008).
• Theodorakis, K., Katidou, M., Doulgeraki, K., Gavalas, A. and Karagogeos, D. Study of the bHLH gene Nscl1 in the vertebrate hindbrain. Hellenic Society of Biochemistry and Molecular Biology, 59th Annual Meeting (2007).
• Katidou, M., Theodorakis, K., Renieri, E., Kalantzaki, M., Gavalas, A. and Karagogeos, D.

The bHLH gene Nscl1 in the developing nervous system: expression and functional studies.

Meeting of the International Society for Developmental Neuroscience, Alberta, Canada, August 24-29, 2006. INT J DEV NEUROSCI 24 (8): [téléphone]).

• Katidou, M., Theodorakis, K., Simigdala, N., Renieri, E. and Karagogeos, D. Expression and functional studies of the neuronal bHLH gene Nscl1. Hellenic Society for Neuroscience, 20th Annual Μeeting (2006).
• Vidaki, M., Denaxa, M., Kyriakopoulou, K., Theodorakis, K., Watanabe, K. and Karagogeos, D. The adhesion molecule TAG-1 is required for the proper migration of neurons of the precerebellar nuclei. Meeting on Axon Guidance, Synaptogenesis & Neural Plasticity, Cold Spring Harbor, New York, September 13-17, (2006).
• Theodorakis, K., Katidou, M., Sabbaki, M., Renieri, E., Kalantzaki, M., Gavalas, A. and Karagogeos, D. The bHLH gene Nscl1 in the developing nervous system. EMBO course on Molecular Mechanisms of Development. Barcelona, Spain, July 3-8, (2006).
• Karagogeos, D., Denaxa , M., Kyriakopoulou , K., Theodorakis, K., Trichas, G., Vidaki, M., Tzimourakas, A., Takeda , Y., Watanabe, K. (2005) Analysis of the phenotype of mice deficient in the neural adhesion molecule TAG-1 during development and in the adult. Soc For Neurosci Conference, Washington DC, (2005).
• Denaxa, M., Kyriakopoulou, K., Vidaki, M., Trichas, G., Theodorakis, K., Petsakou, A., Giasemi, S., Takeda, Y., Watanabe, K., Karagogeos, D. The adhesion molecule TAG-1 is required for the proper migration of neurons of the precerebellar nuclei. ". Hellenic Society for Neuroscience, 19th Annual Meeting (2005).
• Theodorakis, K., Katidou, M., Sabbaki, M., Renieri, E., Kalantzaki, M., Gavalas, A. and Karagogeos, D. The bHLH gene NSCL1 in the developing nervous system. Hellenic Society for Neuroscience, 19th Annual Meeting (2005).
• Theodorakis,K., Wassef,M. and Karagogeos,D. The bHLH gene NSCL1 is expressed in the developing nervous system. Hellenic Society of Biochemistry and Molecular Biology, 53th Annual Meeting (2001).

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• Pavlou, O., Faivre-Sarrailh, C., Theodorakis, K., Rougon, G., Karagogeos, D. "Functional analysis of the neuronal cell adhesion molecule TAG-1 ". Hellenic Society for Neuroscience, 15th Annual Meeting, Patra (2000).
• Denaxa, M., Pavlou, O., Papadopoulos, G.C., Tsiotra, P., Liapaki, K., Theodorakis, Κ., Papamatheakis, J., and Karagogeos, D. Upstream regulatory sequences of the human TAX- 1 gene drive specific expression in the nervous system. Hellenic Society for Neuroscience, 14th Annual Meeting (1999).
• Theodorakis, K. and Karagogeos, D. Molecular analysis and expression pattern of the neuronal bHLH transcripton factor Nscl1. Hellenic Society of Biochemistry and Molecular Biology, 51th Annual Meeting (1999).
• Pavlou, O., Theodorakis, K., Faivre-Sarrailh, C., Rougon, G., Karagogeos, D. "Functional dissection of the human homolog of the axonal glycoprotein TAG-1 in the promotion of neurite outgrowth", Fifth I.B.R.O. World Congress of Neuroscience, (1999).
• Pavlou, O., Denaxa, M., Papadopoulos, G.C., Tsiotra, P., Liapaki, K., Theodorakis, K., Papamatheakis, J., Karagogeos, D. "Upstream regulatory sequences of the human TAX-1 gene drive specific expression to the developing mouse central nervous system", Proceedings of the Society for Neuroscience, 24:1037, (1998).
• Kastrinaki,M.C., Mankoo,B., Theodorakis,K., Pachnis,V. and Karagogeos,D. Identification of the zebrafish mox-1 gene and characterization of its expression in normal, ntl, fth and spt animals. Somite meeting, Les Empiez, France, (1997).
• Tsiotra, P.C., Theodorakis, K., Papamatheakis, J. and Domna Karagogeos. The fibronectin domains of the neural adhesion molecule TAX-1 are necessary and sufficient for homophilic binding. Hellenic Society for Neuroscience, 12th Annual Meeting (1996).
• Tsiotra, P.C., Papamatheakis, J., Theodorakis, K., and Karagogeos, D. Homophilic binding of the neural adhesion molecule TAX-1 is mediated through its fibronectin domains.

Proceedings Soc. Neuroscience, 22:1713, (1996).

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